Utilize the national great science project to promote structural biological

Utilize the national great science project to promote structural biological study
It is reported recently, the national scientific centre (raise) of the light source of Shanghai Director Xu HongJie the radiation device represents simultaneously with He JianHua, Beijing Dong YuHui,etc. radiates experts synchronously, and Shanghai of Chinese Academy of Sciences grows department's institute one is glorious, , it is altogether, it assembles to be here Israel by more thanth honoured guest" Utilize the source of Shanghai to promote structural biological study " It is the 160th Shanghai eastern scientific and technological forum of the theme, discuss together how to " utilize the light source of Shanghai to promote structural biological study " .

In recent years, very nuclear biomembrane albumen crystal structure has been paid close attention to more and more by people. Institute big associate professor Wang Jia of department of university student of Tsing-Hua University says: "Though very nuclear biomembrane albumen is very important, because its one's own characteristic, make the analysis of structure face a lot of technical challenges. " Very nuclear biomembrane albumen develops through the long-term evolution, the function is more single-minded, usually exist only in phosphatide environment steadily. It is not stability and crystallization especially favorable to very nuclear biomembrane albumen to be generally used in the crystalline stain-remover environment of original nuclear membrane albumen at present. Utilize Lipidiccubicphase ' Cubic looks of lipoprotein) The crystalline method has already been verified and has been used in some real cores biological membrane protein crystallization effectively. But this kind of method obtains small crystal measurement, and Lipidiccubicphase will make the opaqueness after cooling at low temperature, orient and bring the trouble for the crystal.

In the face of the above difficulties, Wang JiaWei has proposed needing to build the light beam thread of small yardstick, and utilize the preliminary diffraction picture to make a reservation and to the technology hitting the crystal. He says these technological break-throughs will become preconditions that the albumen structure of very nuclear biomembrane will be succeeded in analyzing in the future.

Earnest hope and requirement in the face of the biological researchers of numerous structure of our country, Shanghai the intersection of light source and the first 7 the intersection of light beam and line stand and can't meet the demand that users increase day by day far already. How utilize and give play to the intersection of Shanghai and excellent characteristic of light source fully, development suitable for high light the intersection of light source and protein crystal structure of characteristic determine that tests the method, become the urgent task faced in next development of the light source of Shanghai.

Shanghai light source national scientific in the center ' Raise) Director Xu HongJie says: "We make worried country worried, national big the intersection of science and the intersection of project and preliminary design of ' the intersection of protein and the intersection of scientific research and the intersection of Shanghai and facility ' adopt, evaluate already, will go into operation soon and build. " It is reported this project will newly build the protein crystallography line stations of three different characteristics on the light source of Shanghai, determine to little crystal structure separately, big brilliant the intersection of afterbirth and protein complex structure determine and the increasingly automated crystal structure is determined. The intersection of protein and little crystal structure to can determine size micron of protein crystal structures in main goal that line stand among them.

It is the construction in the biological application of light source of the third generation of synchronous radiation in the world at present that it is focused on technology that the light beam is detailed and studies one of the focuses. It says what builds researcher Hua in the light source of Shanghai: "Utilize thinly to focus on X ray bunch and launch the structure to study to small crystal, can reduce the requirements for crystal size greatly. " He say Shanghai light source to detailed to focus light beam on, carry on structure examine regular some question too at the same time, for example how to obtain detailed focus light beam of stable, high quality and set up corresponding high precision to measure the device and continue exploring that studies on the premise of keeping high light; Explore small the intersection of crystal and the intersection of diffraction and collection and treatment of data at the same time ' Including amalgamation, collecting the tactics of many drops of crystal diffraction data is chosen) Treatment and the intersection of mechanism and research and small the intersection of crystal and the intersection of diffraction and the intersection of obtaining and analysis of structure of phase place,etc. and problem that radiation damage.

Meanwhile, structural biologist radiate the light source to whom 21st century show up prominently synchronously while being the fourth generation of - -Free electron laser (XFEL) Entertain very great expectation and prospect, X ray free electron laser of U.S.A. SLAC has already produced light, Germany, Japan, South Korea,etc. have already started. In conference's speech, is engaged in for a long time mere researcher Zhang Rong of synchronous radiation and structural biological study introduces: The fourth generation of light source compare the third generation of synchronous radiation, have better intensity, higher coherence and shorter pulse. It is very good to utilize coherence, the free electron laser beam of very big intensity shines the molecule, note the relevant intensity that scatters, can get the structure of the son in Central Plains of molecule, in this way the molecule does not need long crystal, solves biological macromolecule especially crystalline difficult problem of the membrane albumen. The extremely quick pulse can get enough information before the molecule is destroyed by the high-energy at the same time.

Certainly, the fourth generation of light source face a lot of difficult problem still in practical application, survey question, how about win single member to scatter difficult problems such as signals,etc.. Zhang Rong only says: "The application prospect of the fourth generation of light source is very good, but it has much difficulties to be overcome technically, it is still relatively far from practical application. Our country should study, radiate the light source synchronously while being the fourth generation of as soon as possible, otherwise lag behind the other countries afterwards. " (yellow hard land ocean)

Utilize the national great science project to promote structural biological study