It induces the multi-functional stem cell to reproduce the new

It induces the multi-functional stem cell to reproduce the new method to make
It is reported according to U.S.A.'s daily scientific website's recent report, a new method of American scientist's invention, can produce one through mixing two albumens together " The super albumen " ,Thus has improved and made and induced the multi-functional stem cell (iPS) greatly Efficiency sum purity,and production the risks of tumour. Study the thesis to issue on latest " stem cell " magazine.

At present, the scientists mainly introduce four definite genes to make iPS cells through cell of an adult in North Korea, these genes will grow up cells will be programmed as a stem cell again, in order to divide into many kinds of different kinds of cells in the human body. Generally speaking, 4 genes introduced are Oct4, Sox2, Klf4 and c-Myc, so, this kind of method is abbreviated as OSKM. The challenge that OSKM method faces is: In programme in the course again while being whole, only few 0.1% cells really turn into iPS cells; In addition, because c-Myc introduced is the oncogene, may produce tumour.

The new method that the scientific research group of the rich leader of clear peaceful well of interconnected system of Radix Platycodonis of molecule biologist of the research institute of stem cell of Minnesota university invents is: The passage which regulate the factors MyoD of duplicating multi-functional regulation genetic Oct4 and muscle is mixed together, produce one that can improve iPS manufacture process from many aspects " The super gene " M3O (or " super Oct4 " ),Can raise efficiency and purity of making iPS greatly. The scientists can thereby know the mechanism of making iPS cells clearly too.

Radix Platycodonis interconnected system clear to say compared with OSKM method, new method make mouse and the intersection of iPS and efficiency of cell of people, increase by 50 times; The purity is improved to some extent too - -IPS cells produced newly account for 98% of cells got, the purity that and OSKM method is received is only 5%; New method let, recombinate course, become simple too, only needing iPS cells for 5 days will longer than, but use OSKM to take about 2 weeks. Moreover, the new method does not involve c-Myc, has not produced the risk of tumour.

In addition, in OSKM method, human iPS cells usually need and raise cells (the cells used for maintaining embryo's not division state of stem cell in order to amplify enough quantity) Combine and train, raise cells and generally come from mouse's cells, obviously, such a cell is used in the human body transplant and will produce the question. The new method does not need such raising cells, has simplified and fostered the course entirely greatly.

The clear interconnected system of Radix Platycodonis shows, the new method will accelerate making the speed of iPS cells for the particular patient greatly, make cells transplant the treatment and succeed in serving disease as soon as possible.

Also researchers are trying to turn a kind of cell into another kind of cell directly through programming again, thus has wound this stage of iPS cell, for example, through introducing several kinds of genes, induce skin cells to turn into nerve cell,etc.. This kind is called method to programme directly and thought to surmount iPS cell technology and become and make monobasic stem cell's technology. And this new method is expected to be applied to programming on the method directly extensively.

(Liu Xia)

It induces the multi-functional stem cell to reproduce the new method to make