Thesis author: RobertECampbell periodical: <
With the completion of mankind and a lot of other species genome examining order, biologists begin to study that transfers to and adjusts and controls the expression how the gene opens and closes the function gene to have especially, the needing depending on the invention application of new technology and new tool of this kind of research. The near future yes <
Two are new that the biologists in the national high magnetic laboratory of U.S.A. Michael and Davidson and Hazelwood and researcher of Alberta university Teng of Chris create together Fluorogenic for albumen for biology sensor,this kinds of molecule signal people can clear whether distinguish at cell life activity ing. The biology sensor can be used for measuring two independent dynamic activities in a cell at the same time, this and how enzyme is for understanding different albumens work together and finish the daily function, help cells to grow and split up and play a key role. The application of this biology sensor, can help researchers to carry on further investigation on tumour and subject of the development biological field.
The appearance of the new biology sensor makes fluorogenic resonance energy transfer technology (FRET) Have been improved. It refers to energy to transfer from a fluorogenic base group stimulated to the physical phenomenon of another kind of fluorogenic base group in such a manner that it is not radiation that fluorogenic resonance energy is shifted, the energy of FRET shifts efficiency and the distance is closely related with between two fluorogenic base groups, so can be used for determining the distance among the atoms and among the molecules. This characteristic makes FRET technology play an important role in research such as interacting, cell signal thorough fare among macromolecule conformation change, macromolecule, become the important method in biomedical research.
But because the biological process in cells often involves the interaction among the macromolecules of more than two, past FRET technology is often unable to meet biological study's demands. Davidson, leader of research group, says, in fluorogenic resonance energy is shifted, will transmit energy in intravital cells at the time of two fluorogenic molecule interaction, can be observed under the microscope as molecule mark their activity, but we can only observe a biology sensor once all the time.
New technology - -Bilateral FRET is different, it can pass the above-mentioned new biology sensor molecule, measure middle peptide chain and measure two fluorogenic albumens at the same time. New biology sensor based on purple to stimulate getting yellow fluoro outside and getting green a new one fluorogenic albumen gene invented, because they are stimulated the characteristic uniquly, it can be monitored while other members exist. Hundreds of this kind of new biology sensors have already been used for studying the scale type death of cell in the past several years, a series of cell life activity that new Chen DaiXin, cell division, hormone stimulate,etc..
Meanwhile, Davidson group and cooperators further expanded the ability of the optical microscope, they have created the new fluorogenic albumen and observed the method, make the albumen more steady under the microscope. The albumen is very sensitive to the light, will be bleached and disappear after regular hour. Through make albumen to be steady, microscope user can a longer time observe intravital cells.
Synthesize the above-mentioned progress of two items of technology together, can observe two pieces of motive force course of a cell in disposable longer time, it is expected to study with higher speed to go on and expand the application of the optical microscope. (Source: Science and Technology Daily Zhang JiaXing)
Utilize the fluorogenic albumen to spy out the gene to adjust and control
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