The pathogenic gene of little inborn head deformity can be

The pathogenic gene of little inborn head deformity can be clicked as the chemotherapy target
Little inborn head deformity (Primarymicrocephaly) that it is reported It is a kind of rare neural, it was abnormal to impel patient's head to be unusually young that this kind of took shape and grew because of the brain. The child who suffers from this kind of disease may be very short and small and breaking out with the epilepsy, and will present the intelligence obstacle of a certain degree.

Supported by national natural science fund,etc., the teacher-training university students in the capital order the scientific institute's professor to let to revitalize intelligently the seminar find the pathogenic gene of the odd-shaped disease of the inborn small head after further investigating, the mutation of CDK5RAP2 as one of pathogenic gene causes the body of spindle to assemble the non-performance clicked in test, it may be odd-shaped pathogenic molecular mechanism of the small inborn head. The function target that CDK5RAP2 may also be the chemotherapy medicine is clicked. This achievement is issued on the major magazine " cell cycle " studied cell cycle published on April 15.

Xu Xing explains intelligently: Must guarantee and it is single to dye the sisters duplicating it etc. in the normal cell division to give two sub cells, this kind guarantees to be assembled and examines some to finish by the body of spindle. Inactivation that and this test clicks will cause the unstability of chromosome in cells, cause the canceration finally.

Xu Xing is found under study for action intelligently, if inhibit causing the body of spindle to assemble the function clicked in test and lack to the expression of CDK5RAP2, inactivation causing this test to click. "Its molecular mechanism mainly has two points: CDK5RAP2 is that the body of spindle assembles and examines duplicating regulating the factor forward of some key genetic BUBR1 and MAD2; CDK5RAP2 inhibits the body of spindle and assembles the activation which examines some target albumens directly. " Xu Xing infers intelligently.

The subsequent experiment has proved this inference too. Xu Xing finds intelligently, if interfere with technology's inhibiting the expression of CDK5RAP2 with RNA, will result in having silks to split chromosome of the course and separate the body of defect, spindle and assemble and examine some defects, will be accompanied by the body of spindle and assembled the level which examines some key albumens BUBR1 and MAD2 to reduce at the same time, it is raised on the chromosome too much after activation that CDC20 assembles and examines some on the body of spindle. Adjustment factor of the sex of ones that further study proving too CDK5RAP2 is BUBR1 and MAD2 gene and start the son sharp.

In the following research, Xu Xing finds in surprise intelligently, if it will cause tumour cells to chemotherapy medicine Japanese yew alcohol and mould and plain in A tolerance increase to inhibit the expression of CDK5RAP2. "Choose these two kinds of medicines to carry on the test because original finding, the body of spindle assembles and examines some function targets that is the alcohol of Japanese yew to click, and the chromosome DNA is that the mould and plain function target of A is lighted. " Xing Xu explain alcohol and A mould to lead reason tested into usually Japanese yew intelligently. And conversely, train tumour cells in containing the mould and plain or mould and plain in A cultivation ware of alcohol of Japanese yew and A, the expression of CDK5RAP2 will be inhibited. In addition, express insensitive to siRNA CDK5RAP2 have no intention the intersection of sudden change and physical stamina save phenotype of CDK5RAP2siRNA inhibit from, in the intersection of tumour and cell.

"The above-mentioned result shows, CDK5RAP2 is a mould and plain common medicine target of alcohol of Japanese yew and A; That is to say that can influence the mould plain curative effect to tumour of alcohol of Japanese yew and A to the manipulation that CDK5RAP2 expresses. " Xu Xing says intelligently, " CDK5RAP2 is an unprecedented report as the existence that target is lighted of function in chemotherapy. And the new the intersection of medicine and discovery of target confirm patient to the intersection of chemotherapy and sensitiveness of medicine and it makes corresponding healing solutions to be of great importance in time to clinical early period. "

In fact, the seminar that Xu Xing led intelligently launches the pathogenic gene of little inborn head deformity in the world first to relevant research that cell cycle adjusts and controls. As far back as 2004, he find through influence cell to response that DNA damage to the intersection of cancer and key genetic BRCA1 and adjustment of CHK1 little the intersection of head and the intersection of deformity and the intersection of pathogenic gene one of and MCPH1 inborn, link up little inborn head deformity and cancer for the first time. At it publish thesis it is at " the The Chemicalses biological ", it elect for at week had better thesis and enclose to comment specially, it is offered in medias that a lot of are Chinese and foreign the report. Then, found two pathogenic genetic ASPMs and MCPH1 were orientated as the body of centre hub / spindle very much in cells in 2005 and 2006, the thesis is published on " cell cycle ".

"And then, we hope to get more experimental data in little mouse's experiment made on the living to prove our existing experimental result. " Xu XingZhi says finally. (Chen Chen)
The pathogenic gene of little inborn head deformity can be clicked as the chemotherapy target